Generation of 3D Vascular Organoids Using Human Vascular Endothelial Stem Cells and Functional Assessment of MSCs on Vascular Damage

Diabetic retinopathy (DR), a leading cause of blindness, faces limitations with current treatments and a lack of effective disease models. In this study, we will establish unique vascular endothelial stem cells derived from human ES/iPS cells to construct 3D vascular organoids. By applying stressors such as high glucose and hypoxia, we will develop an innovative model that faithfully replicates the pathology of DR.

Furthermore, we will use this model to evaluate the vascular protective and restorative effects of mesenchymal stem cell (MSC)-derived exosomes, followed by in vivo validation through transplantation into mice. Ultimately, this research will bridge the gap to the preclinical stage for a novel DR therapy utilizing MSC exosomes that targets vascular stabilization.